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permeabilization buffer (Elabscience Biotechnology)

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Elabscience Biotechnology permeabilization buffer
Permeabilization Buffer, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+kit/Foxp3%2FTranscription+Factor+Staining+Kit/pm42909153-604-12-15
Average 94 stars, based on 23 article reviews

permeabilization buffer - by Bioz Stars, 2026-11

94/100 stars

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Article Title: Aged ZnO Nanoparticles Promote Breast Cancer Lung Metastasis by Remodeling the Pulmonary Microenvironment Through the CXCL10/Wnt/β‐Catenin Axis
Article Snippet: Alveolar macrophages and RAW264.7 cells: Cells were processed using the fixation/permeabilization buffer kit (Elabscience, E‐CK‐A109) according to the manufacturer's protocol.

Article Title: Plasmodium PI3K suppresses the externalization of phosphatidylserine on infected erythrocytes
Article Snippet: Afterwards, the cells were fixed with 200 μL of fixation buffer (Elabscience, China, Cat# E-CK-A109) for 20 min at room temperature and permeabilized with 300 μL of permeabilization buffer (Elabscience, China, Cat# E-CK-A109).

Staining:

Article Title: Inverse Vaccine Nano-Mimics: In Situ Antigen-Specific Immune Tolerance Induction for Durable Cardiac Repair Post Myocardial Infarction
Article Snippet: Cells were then surface-stained with fluorophore-conjugated antirat CD4 (E-AB-F1105C, Elabscience, China) and CD25 (PE-30314, Finetest, China) antibodies. .. After surface staining, cells were fixed and permeabilized with Fixation/Permeabilization Concentrate and Permeabilization Buffer (E-CK-A108, Elabscience, China) according to the manufacturer's instructions and intracellularly stained with an anti-rat FOXP3 antibody (E-ABF1351J, Elabscience, China). .. Data were acquired using a NovoCyte flow cytometer.

Article Title: Identification of Active Compounds of Centipeda minima for Potentiating Tumor Immunotherapy in Non-Small Cell Lung Cancer via FANCD2-cGAS/STING/IFN-I Signaling Axis.
Article Snippet: .. For the intracellular factor IFNγ staining, the cells were firstly permeabilized by using the Intracellular Fixation/Permeabilization Kit (Elabscience). .. Flow cytometry was performed and analyzed using a CytoFLEX- S flow cytometer along with CytExpert software (Beckman Coulter, California, USA).

Cell Stimulation:

Article Title: In depth study on the mechanism of Xiao-Chai-Hu-Tang in ameliorating irinotecan-induced intestinal toxicity and enhancing antitumor immunity via intestinal microbiota and fecal metabolomics.
Article Snippet: Irinotecan (CPT− 11) is a cornerstone chemotherapeutic for advanced colorectal cancer (CRC), but its clinical use is limited by dose-dependent gastrointestinal (GI) toxicity.. Xiao-Chai-Hu-Tang (XCHT), a classical traditional Chinese medicine formula, alleviates CPT− 11-induced adverse effects, yet its mechanism remains unclear.. This study established a CPT− 11-induced intestinal toxicity model in CRC rats, using multi-omics analyses (16S rRNA sequencing, fecal metabolomics, SCFA quantification) and an antibiotic depletion model to explore XCHT’s mechanism via the gut microbiota-metabolite axis.